Not a lot, over 100 it's a different game.

At 50 it start showing signs of stress as people that knew everything no longer do so. High performers suffer and start feeling left out, because they lose some autonomy.

It particularly impact those whose scope narrows and they liked the broad scope. Those types thrive in startups, but suffer as more structure is added.

Make sure they are fully utilized and don't get bored or you'll lose them, they like to pick what's important for them to work on.