I made my own 64-byte MS-DOS stub that uses a shorter message "Win32 Only!" instead of the usual "This program can only be run in DOS mode" message. By using the shorter stub, using the secret flag to omit the Rich header, and possibly merging sections together, I can usually get the PE header to fit within 512 bytes, and the linker won't pad out the PE header to 1024 bytes.

A copy can be found at https://github.com/Dwedit/NoCopilotKey/blob/main/stub.bin